Determination of aspirin and salicylic acid in transdermal perfusates.

@article{McMahon1998DeterminationOA,
  title={Determination of aspirin and salicylic acid in transdermal perfusates.},
  author={Gibson P McMahon and Stephen J O'Connor and Desmond J. Fitzgerald and Severine Le Roy and Mary T. Kelly},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={707 1-2},
  pages={322-7}
}
A high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of aspirin and salicylic acid in transdermal perfusates. The compounds were separated on a C8 Nucleosil column (5 microm, 250x4.6 mm) using a mobile phase containing a mixture of water-acetonitrile-orthophosphoric acid (650:350:2, v/v/v) and a flow-rate of 1 ml/min. The transdermal samples were in phosphate-buffered saline (PBS) and could be injected directly onto the HPLC system. The… CONTINUE READING