Determination of asiatic acid in beagle dog plasma after oral administration of Centella asiatica extract by precolumn derivatization RP-HPLC.

@article{Zheng2009DeterminationOA,
  title={Determination of asiatic acid in beagle dog plasma after oral administration of Centella asiatica extract by precolumn derivatization RP-HPLC.},
  author={Xiao-Chun Zheng and Sheng-Hao Wang},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2009},
  volume={877 5-6},
  pages={477-81}
}
A novel precolumn derivatization reversed-phase high-performance liquid chromatography (RP-HPLC) method with UV-vis detection for the quantitative determination of total concentration of asiatic acid (AA) in beagle dog plasma is described. AA was extracted with n-hexane-dichloromethane-2-propanol (20:10:1, v/v/v) from plasma, which had been hydrolyzed by acid and derivatized with p-Toluidine. Chromatographic separation was achieved on a C(18) column using gradient elution in a water-methanol… CONTINUE READING

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