Determination of anti-giardial activity in vitro by means of soluble formazan production.

@article{Wright1992DeterminationOA,
  title={Determination of anti-giardial activity in vitro by means of soluble formazan production.},
  author={Colin W. Wright and S I Melwani and J. David Phillipson and David Charles Warhurst},
  journal={Transactions of the Royal Society of Tropical Medicine and Hygiene},
  year={1992},
  volume={86 5},
  pages={517-9}
}
A new microplate assay for the determination of activity in vitro against Giardia intestinalis has been developed in which viability is measured by soluble formazan production from a tetrazolium reagent. Metabolic reduction of the latter gives rise to a coloured product which may be determined directly by optical density measurement. Using this method the anti-giardial activities of more than 20 anti-microbial compounds have been assessed. The results obtained with a number of known anti… CONTINUE READING