Determination of alpha-tocopherol in erythrocytes by gas-liquid chromatography.

@article{Bieri1970DeterminationOA,
  title={Determination of alpha-tocopherol in erythrocytes by gas-liquid chromatography.},
  author={John G. Bieri and R K Poukka and E L Prival},
  journal={Journal of lipid research},
  year={1970},
  volume={11 2},
  pages={118-23}
}
A method is described for the determination of submicrogram amounts of alpha-tocopherol in 0.5 ml of packed erythrocytes. The alpha-tocopherol in a lipid extract is oxidized to alpha-tocopherylquinone which is separated by thin-layer chromatography, eluted, and quantitated by gas-liquid chromatography. Calculation is based on the recovery of added alpha… CONTINUE READING