Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction.

OBJECTIVE To confirm alpha-1-AT deficiency status in duodenal ulcer using a combination of PCR and restricted enzyme digestion. METHODS Fifty patients with endoscopically proven duodenal ulcer and hundred controls with no signs of the disease were included. Alpha-1-AT phenotypes were confirmed by polymerase chain reaction followed by restriction enzyme… (More)