Determination of aflatoxin B1 in corn, wheat, and peanut butter by enzyme-linked immunosorbent assay and solid phase radioimmunoassay.

@article{ElNakib1981DeterminationOA,
  title={Determination of aflatoxin B1 in corn, wheat, and peanut butter by enzyme-linked immunosorbent assay and solid phase radioimmunoassay.},
  author={O El-Nakib and James J. Pestka and Fun Sun Chu},
  journal={Journal - Association of Official Analytical Chemists},
  year={1981},
  volume={64 5},
  pages={1077-82}
}
Determinations of aflatoxin B1 in corn, wheat, and peanut butter by an enzyme-linked immunoassay (ELISA) and a solid-phase radioimmunoassay (RIA) were compared. Samples spiked with 2.9-43.2 ppb B1 were subjected to AOAC extraction procedure 26.017 or 26.023. The extracts were concentrated, redissolved in methanol, diluted in phosphate-buffered saline with Tween 20, and directly analyzed for B1 by either ELISA or RIA. At 5.8 ppb or greater, recoveries for B1 in corn, wheat, and peanut butter… CONTINUE READING