Determination of aconitine in body fluids by LC-MS-MS

  title={Determination of aconitine in body fluids by LC-MS-MS},
  author={Justus Beike and Lara Frommherz and Michelle Wood and Bernd Brinkmann and Helga K{\"o}hler},
  journal={International Journal of Legal Medicine},
A very sensitive and specific method was developed for the determination of aconitine, the main toxic alkaloid from plants of the genus Aconitum L., in biological samples. The method comprised solid-phase extraction using mixed-mode C8 cation exchange columns followed by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Chromatographic separation was achieved with a RP8 column. Detection of aconitine was achieved using electrospray in the positive ionisation mode and quantification was… 
Quantitative determination of taxine B in body fluids by LC–MS–MS
A new specific and sensitive LC–MS–MS method for the detection of taxine B and isotaxine B, the main toxic pseudo-alkaloids from yew (Taxus sp.), in biological samples (blood, urine, gastric content) was developed and applied in four fatal cases with suspected yew intoxication.
Development of a novel HPLC-MS/MS method for the determination of aconitine and its application to in vitro and rat microdialysis samples.
Following characterization of aconitine both in vitro and in vivo microdialysis, the developed setting is suitable for application in pharmacokinetics and pharmacodynamics studies.
Simultaneous analysis of aconitine, mesaconitine, hypaconitine, and jesaconitine in whole blood by LC-MS-MS using a new polymer column
The present LC-MS-MS method is very effective for simple simultaneous analysis of aconitine and its analogues in forensic and clinical toxicology.
Simultaneous determination of three aconitum alkaloids in urine by LC-MS-MS.
Detection and validated quantification of toxic alkaloids in human blood plasma--comparison of LC-APCI-MS with LC-ESI-MS/MS.
Both presented assays were applicable for sensitive detection of all studied analytes and for accurate and precise quantification, with the exception of the rather volatile nicotine.
[Simultaneous determination of aconitine analogues in Aconitum plants and foods that caused food poisoning by liquid chromatography with tandem mass spectrometry].
Results indicate that the developed method is suitable for the determination of aconitine analogues in Aconitum plants and foods that cause food poisoning.
Quantification of aconitine in post-mortem specimens by validated liquid chromatography-tandem mass spectrometry method: three case reports on fatal 'monkshood' poisoning.
The diester-diterpene alkaloid aconitine was quantified by liquid chromatography-tandem mass spectrometry in post-mortem specimens of three cases where suicidal ingestion of Aconitum napellus L. ('monkshood') was supposed to provide compelling evidence for acute Aconite poisoning as being the cause of death.
Rapid determination of yunaconitine and related alkaloids in aconites and aconite-containing drugs by ultra high-performance liquid chromatography-tandem mass spectrometry.
A rapid and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) detection combined with microwave-assisted extraction (MAE) was developed for high throughput simultaneous determination of YAC and six other toxic aconite alkaloids in 31 samples of crude, processed aconites and aconite-containing drugs.
Hidden aconite poisoning: identification of yunaconitine and related aconitum alkaloids in urine by liquid chromatography-tandem mass spectrometry.
Yunaconitne, a highly toxic aconitum alkaloid, was thus identified in human urine for the first time and is an important diagnostic aid for acute poisoning by aconites of an unclear origin.


LC-MS determination of Taxus alkaloids in biological specimens
This method was used to examine the cause of death of a 43-year-old man who died several hours after he drank a decoction of taxus leaves, and is the first case in which a semi-quantitative analysis of taxine alkaloids has been performed.
Electrospray ionization tandem mass spectrometric study of the aconitines in the roots of aconite.
All the known aconitines were detected and also 23 unknown ac onitine-type alkaloids, in which the lipo-alkaloids containing residues of 15C, 17C and 19C saturated or unsaturated fatty acids were characterized, have not been reported previously.
Method for the simultaneous determination of Aconitum alkaloids and their hydrolysis products by gas chromatography-mass spectrometry in human serum.
This paper describes a newly developed method using gas chromatography-selected ion monitoring (GC-SIM) for the simultaneous determination of diester-diterpene type Aconitum alkaloids and their
Quantitative analysis of Aconitum alkaloids in the urine and serum of a male attempting suicide by oral intake of aconite extract.
The urine was a useful material to identify the toxicants in the case of aconite intoxication and some alkaloids were still detectable in the urine even six days after intoxication.
Characterization of aconitine-type alkaloids in the flowers of Aconitum kusnezoffii by electrospray ionization tandem mass spectrometry.
A rapid method for the direct detection and characterization of alkaloids from an ethanolic extract of FAK is described, and all the known aconitum alkaloid are detected and a series of lipo-aconitines has been found for the first time in this plant.
Distribution of Aconitum alkaloids in body fluids and tissues in a suicidal case of aconite ingestion.
Findings show that Aconitum alkaloids were found in the liver and kidneys in much higher concentrations than in serum and suggest that they were eliminated not only via urine but also in feces.
A case of aconitine poisoning with analysis of aconitine alkaloids by GC/SIM.
Exploring the ester-exchange reactions of diester-diterpenoid alkaloids in the aconite decoction process by electrospray ionization tandem mass spectrometry.
Electrospray ionization tandem mass spectrometry was used to explore the ester-exchange reactions for aconitine-type diester-diterpenoid alkaloids occurring during the process of decocting aconite root, and it was found that diedter- diterpenoids were converted into lipo-alkaloids as well as monoester alkal steroids by the decoction of aconite.