Determination of acid phosphatase in biological fluids using a new substrate, 2,6-dichloro-4-nitrophenyl phosphate.

@article{Teshima1987DeterminationOA,
  title={Determination of acid phosphatase in biological fluids using a new substrate, 2,6-dichloro-4-nitrophenyl phosphate.},
  author={S. Teshima and Y. Hayashi and M. Ando},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1987},
  volume={168 2},
  pages={
          231-8
        }
}
  • S. Teshima, Y. Hayashi, M. Ando
  • Published 1987
  • Chemistry, Medicine
  • Clinica chimica acta; international journal of clinical chemistry
  • A new substrate, 2,6-dichloro-4-nitrophenyl phosphate (DCNP-P), is used for the determination of acid phosphatase (EC 3.1.3.2) in serum and urine. It was hydrolyzed by acid phosphatase to 2,6-dichloro-4-nitrophenol (DCNP) and phosphoric acid. At a pH of 4.5-6.0, the absorption of DCNP liberated by acid phosphatase was much higher than that of p-nitrophenol, which is commonly used as an aglycone in the acid phosphatase assay. By using DCNP-P as a substrate for acid phosphatase activity… CONTINUE READING
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