Determination of acetaminophen-protein adducts in mouse liver and serum and human serum after hepatotoxic doses of acetaminophen using high-performance liquid chromatography with electrochemical detection.

@article{Muldrew2002DeterminationOA,
  title={Determination of acetaminophen-protein adducts in mouse liver and serum and human serum after hepatotoxic doses of acetaminophen using high-performance liquid chromatography with electrochemical detection.},
  author={Kenneth L Muldrew and Laura P. James and Leslie Coop and Sandra S. McCullough and Howard P. Hendrickson and Jack A. Hinson and Philip R. Mayeux},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2002},
  volume={30 4},
  pages={446-51}
}
Acetaminophen-induced hepatotoxicity has been attributed to covalent binding of the reactive metabolite N-acetyl-p-benzoquinone imine to cysteine groups on proteins as an acetaminophen-cysteine conjugate. We report a high-performance liquid chromatography with electrochemical detection (HPLC-ECD) assay for the conjugate with increased sensitivity compared with previous methods. Previous methods to quantitate the protein-bound conjugate have used a competitive immunoassay or radiolabeled… CONTINUE READING

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