Determination of absolute protein numbers in single synapses by a GFP-based calibration technique

@article{Sugiyama2005DeterminationOA,
  title={Determination of absolute protein numbers in single synapses by a GFP-based calibration technique},
  author={Yoshiko Sugiyama and Izumi Kawabata and Kenji Sobue and Shigeo Okabe},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={677-684}
}
To build a quantitative model of molecular organization of neurons, it is essential to have information about the number of protein molecules at individual synapses. Here we developed a method to estimate absolute numbers of individual proteins at actual excitatory synapses by calibrating the fluorescence intensity of microspheres with single EGFP molecules. In cultured hippocampal neurons, we observed a monotonous increase of postsynaptic protein numbers per single synapse during neuronal… CONTINUE READING

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