Determination of a protein ligand interaction via continuous isothermal titration calorimetry

@inproceedings{Demarse2011DeterminationOA,
  title={Determination of a protein ligand interaction via continuous isothermal titration calorimetry},
  author={Neil A. Demarse and Colette F. Quinn},
  year={2011}
}
Isothermal titration calorimetry (ITC) is a straightforward method to determine basic chemical details of a binding interaction (affinity, thermodynamics and stoichiometry) in a single experiment and under native conditions [1, 2]. Traditionally, ITC experiments are performed using the method of incremental titration, whereby a precise volume of titrant is added to a solution of titrand at discrete time-intervals (Figure. 1). The area under each peak is then integrated, normalized and fit to a… CONTINUE READING

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