Determination of a novel beta-lactam antibiotic (E-1100) in rat plasma by high-performance liquid chromatography.
@article{Tokumura1993DeterminationOA,
title={Determination of a novel beta-lactam antibiotic (E-1100) in rat plasma by high-performance liquid chromatography.},
author={Tadakazu Tokumura and Toru Horie},
journal={Journal of chromatography},
year={1993},
volume={620 1},
pages={
153-7
}
}A fast, simple and accurate method to determine the concentration of E-1100, a novel beta-lactam antibiotic, in rat plasma has been developed. This method involves deproteinization by methanol with 0.2% perchloric acid and reversed-phase high-performance liquid chromatography with ultraviolet detection. Regression analysis showed that the method was linear over the standard-curve range from 0.191 to 191.0 micrograms/ml. This newly developed method has been applied for the analysis of plasma…