Determination of Urea in Blood and Urine with Diacetyl Monoxime-glucuronolactone Reagent.

@article{Momose1965DeterminationOU,
  title={Determination of Urea in Blood and Urine with Diacetyl Monoxime-glucuronolactone Reagent.},
  author={Tsuyoshi Momose and Yasufumi Ohkura and J Tomita},
  journal={Clinical chemistry},
  year={1965},
  volume={11},
  pages={113-21}
}
N UMEROUS STUDIES have been reported in the literature on the photometric determination of urea in biological fluids with diacetyl monoxime as the color-developing agent. The reaction is photosensitive, does not follow Beer’s law, and requires special precautions for reproducible results. In many proposed procedures, oxidants were added in the reaction mixture to destroy hydroxylamine which might be generated during the reaction by the strong acidity, and decrease the produced color. The… CONTINUE READING