Determination of Sulfate-Conjugated 3β-Hydroxy Bile Acid in Urine by HPLC with Postcolumn Fluorescence Detection Using an Immobilized β-Hydroxysteroid Dehydrogenase Column

  title={Determination of Sulfate-Conjugated 3$\beta$-Hydroxy Bile Acid in Urine by HPLC with Postcolumn Fluorescence Detection Using an Immobilized $\beta$-Hydroxysteroid Dehydrogenase Column},
  author={K. Kuruma and Y. Morioka and K. Sasakura and H. Igimi and K. Uchida},
  journal={Analytical Sciences},
A highly sensitive and specific method was established for quantification of 3β-hydroxy-5-cholenoic acid by high- Performance liquid chromatography using an immobilized -β-hydroxysteroid dehydrogenase column (β-HSD column) and a postcolumn fluorescent detection. The β-HSD column shows maximum enzyme activity and stability at 20°C, and has a very short life at 25°C. The detection limit was 7pmol/injection. We also developed an efficient method for extracting the sulfate conjugates at position 3… Expand
4 Citations
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We developed a highly sensitive and quantitative method to detect bile acid 3-sulfates in human urine employing liquid chromatography/electrospray ionization-tandem mass spectrometry. This methodExpand
Biological fluid screening and confirmation of bile acids by use of an integrated flow-injection-LC-evaporative light-scattering system
SummaryAn integrated flow-injection-liquid chromatographic system is presented for the screening of biological fluids for bile acids, and for subsequent confirmation. It is based on the use of aExpand
Transformation of bile acids and sterols by clostridia (fusiform bacteria) in Wistar rats
After inoculation of clostridia into germ-free rats and into rats previously inoculated solely with Escherichia coli, most of the endogenous bile acids were deconjugated, and cholic acid and chenodeoxycholic acid were 7α-dehydroxylated to deoxycholics acid and lithocholic acid, respectively. Expand
A novel immobilization fluorescence capillary analysis method and its applications.
The immobilized FCA method has been successfully used in the determination of reduced coenzyme I, ethanol in liqueur, lactic acid in dairy products,pyruvic acid and glucose in serum, trace-level sulfated bile acid in urine, the ratio of pyruvic/lactic acidIn serum, and pyru Vic acid in cells as well as in DNA end-labeling and dyeing methods. Expand