Determination of Sulfate-Conjugated 3β-Hydroxy Bile Acid in Urine by HPLC with Postcolumn Fluorescence Detection Using an Immobilized β-Hydroxysteroid Dehydrogenase Column

@article{Kuruma1996DeterminationOS,
  title={Determination of Sulfate-Conjugated 3$\beta$-Hydroxy Bile Acid in Urine by HPLC with Postcolumn Fluorescence Detection Using an Immobilized $\beta$-Hydroxysteroid Dehydrogenase Column},
  author={K. Kuruma and Y. Morioka and K. Sasakura and H. Igimi and K. Uchida},
  journal={Analytical Sciences},
  year={1996},
  volume={12},
  pages={847-851}
}
A highly sensitive and specific method was established for quantification of 3β-hydroxy-5-cholenoic acid by high- Performance liquid chromatography using an immobilized -β-hydroxysteroid dehydrogenase column (β-HSD column) and a postcolumn fluorescent detection. The β-HSD column shows maximum enzyme activity and stability at 20°C, and has a very short life at 25°C. The detection limit was 7pmol/injection. We also developed an efficient method for extracting the sulfate conjugates at position 3… Expand
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