Determination of RS,E/Z-tocotrienols by HPLC.

  title={Determination of RS,E/Z-tocotrienols by HPLC.},
  author={A M Drotleff and Waldemar Ternes},
  journal={Journal of chromatography. A},
  volume={909 2},
Synthetic alpha-tocotrienol was separated into four geometrical E/Z side chain isomers by preparative HPLC (permethylated beta-cyclodextrin phase). The isolated isomers were resolved in ethylene glycol dimethyl ether, converted into the corresponding methyl ether using dimethyl sulfate, and the tocotrienol methyl ethers were extracted with n-hexane. A subsequent HPLC separation on a chiral phase (adsorbent cellulose derivated with 3,5-dimethyl phenyl carbamate) discriminates between the… CONTINUE READING