Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

@inproceedings{Vivoli2014DeterminationOP,
  title={Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry},
  author={Mirella Vivoli and Halina R. Novak and Jennifer A. Littlechild and Nicholas J Harmer},
  booktitle={Journal of visualized experiments : JoVE},
  year={2014}
}
A wide range of methods are currently available for determining the dissociation constant between a protein and interacting small molecules. However, most of these require access to specialist equipment, and often require a degree of expertise to effectively establish reliable experiments and analyze data. Differential scanning fluorimetry (DSF) is being increasingly used as a robust method for initial screening of proteins for interacting small molecules, either for identifying physiological… CONTINUE READING
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