Determination of N-acetylneuraminic acid and N-glycolylneuraminic acid in glycoproteins by high-performance liquid chromatography without derivatization.

Abstract

A simple and sensitive method for the determination of N-acetylneuraminic acid and N-glycolylneuraminic acid in glycoproteins by high-performance liquid chromatography has been investigated. The best condition to release sialic acid from glycoproteins involved incubation at 80 degrees C for 1 h in vacuo in 0.01 M hydrochloric acid with removal of the… (More)

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Cite this paper

@article{Ogawa1993DeterminationON, title={Determination of N-acetylneuraminic acid and N-glycolylneuraminic acid in glycoproteins by high-performance liquid chromatography without derivatization.}, author={Haruo Ogawa and Yasuo Takeuchi and Hiroshi Uchibori and Isao Matsumoto and Nobuko Seno}, journal={Journal of chromatography}, year={1993}, volume={612 1}, pages={145-9} }