Determination of MK-507, a novel topically effective carbonic anhydrase inhibitor, and its de-ethylated metabolite in human whole blood, plasma, and urine by high-performance liquid chromatography.

@article{Matuszewski1994DeterminationOM,
  title={Determination of MK-507, a novel topically effective carbonic anhydrase inhibitor, and its de-ethylated metabolite in human whole blood, plasma, and urine by high-performance liquid chromatography.},
  author={Bogdan K. Matuszewski and Marvin L. Constanzer and E. Woolf and Tsz-Chiu Au and H M Haddix},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1994},
  volume={653 1},
  pages={77-85}
}
Sensitive methods for the determination of a novel topically effective carbonic anhydrase inhibitor (CAI) I, MK-507, and its de-ethylated metabolite II, in human whole blood, plasma and urine were developed. These methods were based on liquid-liquid extraction of I and II from biological matrices, back extraction into acid, and analysis by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection (252 nm). The assays were fully validated in the concentration range of 5 to… CONTINUE READING