Determination of Glutathione and Glutathione Disulfide in Biological Samples Using Acrylonitrile as a Thiol-Blocking Reagent

@article{Matsumoto1996DeterminationOG,
  title={Determination of Glutathione and Glutathione Disulfide in Biological Samples Using Acrylonitrile as a Thiol-Blocking Reagent},
  author={S. Matsumoto and M. Teshigawara and S. Tsuboi and S. Ohmori},
  journal={Analytical Sciences},
  year={1996},
  volume={12},
  pages={91-95}
}
Biological samples containing glutathione (GSH) and glutathione disulfide (GSSG) were deproteinized by HClO 4 or metaphosphoric acid in the presence of bathophenanthroline disulfate. The total glutathione (GSH+2XGSSG) in the sample was determined using 5,5'-dithiobis(2-nitrobenzoic acid) and glutathione reductase by the standard enzymatic recycles methods. GSH was determined as the difference between the concentration of the total glutathione and GSSG ; GSSG was determined after blocking the… Expand
High-performance liquid chromatographic method to analyze picomole levels of glutathione, cysteine and cysteinylglycine and its application to pre-cancerous rat livers.
TLDR
The cysteine level in pre-cancerous livers was extremely elevated in comparison to that of the control groups, while no difference was observed in the glutathione contents between the pre- cancerous and control groups. Expand
HPLC determination of glutathione and other thiols in human mononuclear blood cells.
TLDR
The proposed HPLC method allowed for the separation and quantitation of cysteine and N-acetylcysteine, if present in biological samples, and allows for the determination of total thiol present in human mononuclear cells. Expand
Chemiluminescence Method for the Determination of Glutathione in Human Serum Using the Ru(phen)32+ – KMnO4 System
Abstract.A simple, rapid and specific chemiluminescence (CL) method for the determination of glutathione has been developed. The method is based on the enhanced CL of the reaction between Ru(phen)32+Expand
Mechanism of protection by S-(1,2-dicarboxyethyl)glutathione triester against acetaminophen-induced hepatotoxicity in rat hepatocytes.
TLDR
Treatment with the triester of S-(1,2-dicarboxyethyl)glutathione (DCE-GS) prevented the hepatotoxicity induced by acetaminophen via elevation of the glutathione level in rat hepatocytes through hydrolyzed and produced increased the GSH level via activation of gamma-GCS in rat liver homogenate. Expand
Anti-oxidative Activity of Hydrolysate from Rice Bran Protein in HepG2 Cells.
TLDR
Results indicate that RBPH exerts a protective effect against oxidative stress by modulating GSH levels and anti-oxidative enzyme expression via the Nrf2 pathway. Expand
Changes in levels of glutathione and related compounds and activities of glutathione-related enzymes during rat liver regeneration
TLDR
The enzyme activities of cystathionine-β synthase and γ-cystathionease for cysteine synthesis, and ofγ-glutamylcysteine synthetase, which is a limiting enzyme for glutathione synthesis, were clearly increased in regenerating liver, which could be clearly accounted for by the elevation of these enzyme activities. Expand
Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence.
TLDR
A practical analysis of HCys, GSH, and Glu-cys in human urine sample has been accomplished by this present method using Tween 20-capped gold nanoparticles coupled with laser-induced fluorescence (CE-LIF). Expand
Hypometabolism, antioxidant defenses and free radical metabolism in the pulmonate land snail Helix aspersa
TLDR
The regulation of the antioxidant system during hypometabolism may constitute a mechanism to minimize oxidative stress during cycles of estivation and awakening of Helix aspersa. Expand
Elevation of mitochondrial glutathione by γ‐glutamylcysteine ethyl ester protects mitochondria against peroxynitrite‐induced oxidative stress
TLDR
Results suggest that GCEE may be effective in increasing mitochondrial GSH and may be prove to have therapeutic relevance in neurodegenerative disorders associated with oxidative stress and mitochondrial dysfunction. Expand
Dose-dependent biochemical changes in rat central nervous system after 12-week exposure to 1-bromopropane.
TLDR
The biochemical changes in the cerebrum indicate that long-term exposure to 1-bromopropane has effects on the central nervous system (CNS) of rats and suggests that glutathione depletion and changes in proteins containing SH base as a critical site might be the underlying neurotoxic mechanism of 1-Bromoproane. Expand
...
1
2
3
4
...

References

High-performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives.
Publisher Summary High-performance liquid chromatography (HPLC) provides a rapid, sensitive, and reproducible means of separating and quantifying simultaneously a variety of sulfur-containing aminoExpand