Determination of Glutathione and Glutathione Disulfide in Biological Samples Using Acrylonitrile as a Thiol-Blocking Reagent

  title={Determination of Glutathione and Glutathione Disulfide in Biological Samples Using Acrylonitrile as a Thiol-Blocking Reagent},
  author={S. Matsumoto and M. Teshigawara and S. Tsuboi and S. Ohmori},
  journal={Analytical Sciences},
Biological samples containing glutathione (GSH) and glutathione disulfide (GSSG) were deproteinized by HClO 4 or metaphosphoric acid in the presence of bathophenanthroline disulfate. The total glutathione (GSH+2XGSSG) in the sample was determined using 5,5'-dithiobis(2-nitrobenzoic acid) and glutathione reductase by the standard enzymatic recycles methods. GSH was determined as the difference between the concentration of the total glutathione and GSSG ; GSSG was determined after blocking the… Expand
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The biochemical changes in the cerebrum indicate that long-term exposure to 1-bromopropane has effects on the central nervous system (CNS) of rats and suggests that glutathione depletion and changes in proteins containing SH base as a critical site might be the underlying neurotoxic mechanism of 1-Bromoproane. Expand


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