Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor

  title={Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor},
  author={P. Trinder},
  journal={Annals of Clinical Biochemistry},
  pages={24 - 27}
  • P. Trinder
  • Published 1969
  • Medicine
  • Annals of Clinical Biochemistry
the oxygen acceptors originally used were 0 tolidine, benzidine and o-dianisidine. It has since been established that these three substances are carcinogens and many alternative oxygen acceptors have been suggested. Any dye showing oxidation-reduction properties or any dye formed by oxidation, such as those used in colour photography, are potentially useful but it is obviously advantageous to use reagents which have high stability. For manual work on blood a two-solution technique is desirable… Expand
Interference in glucose oxidase-peroxidase blood glucose methods.
  • P. Sharp
  • Chemistry, Medicine
  • Clinica chimica acta; international journal of clinical chemistry
  • 1972
By utilising other compounds with similar chemical groups, it was found possible to identify the group in the tolazamide molecule that caused interference and consequently to predict the effects of other compounds on such blood glucose methods. Expand
New chromogen for assay of glucose in serum.
A colorimetric assay for glucose determination in human serum, with use of the chromogen 2-amino-4-hydroxybenzenesulfonic acid (AHBS), glucose oxidase, and peroxidase, with results correlated well with those of the phenol/4- aminoantipyrine method of Trinder. Expand
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The results have been used to develop a general kinetic fixed-time method for the analysis of glucose in whole blood and serum and the single reagent method has been adapted to the ENI GEMSAEC centrifugal analyzer and to the Abbott ABA-100 analyzer. Expand
Urinary glycolate measured by use of (S)-2-hydroxy-acid oxidase.
Glycolate can be determined in urine by using (S)-2-hydroxy-acid oxidase immobilized in a continuous-flow system or in a semiautomated procedure for the centrifugal analyzer, which is accurate, precise, and sensitive but unsuitable for routine use because the oxidase is unstable and can only be used for 12 days. Expand
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The spectral properties (absorbance maxima and apparent absorption coefficients) and stability of a large number of chromogenic horseradish peroxidase substrates were surveyed for their ability to perform in an assay for glucose in the presence of phenol red and sera components. Expand
Evaluation of Trinder's glucose oxidase method for measuring glucose in serum and urine.
Trinder's method for glucose has nearly all the attributes of an ideal automated colorimetric glucose oxidase procedure and a study of the method by the simplex technique revealed that the glucose oxidation activity in the reagent is the most critical variable. Expand
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A new enzymic colorimetric method in which urea is measured in serum by use of a single reagent mixture that compared well with those by a candidate Reference Method and are not subject to interferences from commonly used drugs and anticoagulants. Expand
Solid-Phase Determination of Glucose in Whole Blood
Abstract A solid-phase determination of glucose in whole blood has been developed that employs an immobilized substrate and aqueous enzymes. A phenol, 3-hydroxyphenylacetic acid, was covalentlyExpand
Glucose determination in samples taken by microdialysis by peroxidase-catalyzed luminol chemiluminescence.
Glucose added to microdialysate resulted in a complete recovery compared to a H2O2 standard, and glucose values with the present method correlated strongly with values obtained using a routine method involving glucose oxidase and peroxidase. Expand
Determination of glucose by a kinetic method on a thin-layer chromatogram using the oxidation of 3,3′,5,5′-tetramethylbenzidine with hydrogen peroxide
The oxidation of 3,3′,5,5′-tetramethylbenzidine with hydrogen peroxide in aqueous solutions in the presence of iron(II, III) was studied on the surface of chromatography paper and TLC platesExpand