Determination of G-protein-coupled receptor oligomerization by molecular brightness analyses in single cells.

  title={Determination of G-protein-coupled receptor oligomerization by molecular brightness analyses in single cells.},
  author={Ali Işbilir and Robert Serfling and Jan M{\"o}ller and Romy Thomas and Chiara De Faveri and Ulrike Zabel and Marco Scarselli and Annette G. Beck‐Sickinger and Andreas Bock and Irene Coin and Martin J. Lohse and Paolo Annibale},
  journal={Nature protocols},
Oligomerization of membrane proteins has received intense research interest because of their importance in cellular signaling and the large pharmacological and clinical potential this offers. Fluorescence imaging methods are emerging as a valid tool to quantify membrane protein oligomerization at high spatial and temporal resolution. Here, we provide a detailed protocol for an image-based method to determine the number and oligomerization state of fluorescently labeled prototypical G-protein… 
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