Determination of Functional Role of Nucleotide Composition in the Transcription Start Region of the Escherichia coli udp Gene

@article{Ovcharova2003DeterminationOF,
  title={Determination of Functional Role of Nucleotide Composition in the Transcription Start Region of the Escherichia coli udp Gene},
  author={Irina Ovcharova and Svetlana Eremina and Alexander Mironov},
  journal={Russian Journal of Genetics},
  year={2003},
  volume={39},
  pages={256-264}
}
The promoter of the Escherichia coli udp gene contains the poly-T (5"-TTTTT-3") motif in the transcription start region located at the distance of 3 nucleotides with respect to the Pribnow box. By means of site-directed mutagenesis, mutations in the +1, –1, and +3 positions of this region were isolated and their functional role in transcription initiation was determined. It was shown that in addition to the thymine nucleotide earlier identified at position 4 (with respect to the 5" end of the… CONTINUE READING