Determination of Dopamine-β-hydroxylase Activity in Human Serum Using UHPLC-PDA Detection

@article{Punchaichira2018DeterminationOD,
  title={Determination of Dopamine-$\beta$-hydroxylase Activity in Human Serum Using UHPLC-PDA Detection},
  author={Toyanji Joseph Punchaichira and Smita Neelkanth Deshpande and B. K. Thelma},
  journal={Neurochemical Research},
  year={2018},
  volume={43},
  pages={2324-2332}
}
Dopamine-β-hydroxylase (DBH, EC 1.14.17.1) is an enzyme with implications in various neuropsychiatric and cardiovascular diseases and is a known drug target. There is a dearth of cost effective and fast method for estimation of activity of this enzyme. A sensitive UHPLC based method for the estimation of DBH activity in human sera samples based on separation of substrate tyramine from the product octopamine in 3 min is described here. In this newly developed protocol, a Solid Phase Extraction… 

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