Determination of Damaged Starch and Diastatic Activity in Wheat Flour Using a Flow-Injection Analysis Biosensor Method

@article{Haginoya1997DeterminationOD,
  title={Determination of Damaged Starch and Diastatic Activity in Wheat Flour Using a Flow-Injection Analysis Biosensor Method},
  author={Ryuichi Haginoya and Kunio Sakai and Takashi Komatsu and S. Nagao and K. Yokoyama and T. Takeuchi and R. Matsukawa and I. Karube},
  journal={Cereal Chemistry},
  year={1997},
  volume={74},
  pages={745-749}
}
ABSTRACT The determination of damaged starch and diastatic activity in flour was studied using a flow-injection analysis (FIA) biosensor system. The system consisted of an oxygen electrode and an immobilized enzyme column containing purified glucoamylase and glucose oxidase immobilized on activated aminopropyl glass beads. The biosensor system has an optimum pH between 6.5 and 7.5 and an optimum temperature of 35°C for glucose measurement. The response of the FIA biosensor was linear up to 1… Expand
5 Citations
A maltose biosensor for determining gelatinized starch in processed cereal foods
ABSTRACT A reliable method for the quantitative determination of gelatinized starch in processed cereal foods was developed. It consists of an electrochemical biosensor based on amyloglucosidase andExpand
Damaged starch characterisation by ultracentrifugation.
TLDR
The relative molecular size distributions of a selection of starches (waxy maize, pea and maize) that had received differing amounts of damage from ball milling were compared using analytical ultracentrifugation to reflect the effect of lipid-complexed amylose in the maize starch. Expand
Enzymatic conversions of starch.
  • P. Tomasik, D. Horton
  • Chemistry, Medicine
  • Advances in carbohydrate chemistry and biochemistry
  • 2012
TLDR
Methods for the enzymatic conversion of starch, involving hydrolases and nonhydrolyzing enzymes, as well as the role of microorganisms producing such enzymes, are surveyed, covering the period from the early 19th century up to 2009. Expand
Shorter Communication: Grist Fractionation and Starch Modification During the Milling of Malt
Malt is one of the main raw materials in the brewing process and must provide the brewer with the appropriate levels of malt extract easily fermentable by the brewers’ yeast. Starch is a majorExpand
Current research activity in biosensors
TLDR
Current research activity on the fundamentals and applications of biosensors is summarized and molecular recognition elements, techniques and tools for biosensor construction, and basic biosensor devices are introduced. Expand

References

SHOWING 1-5 OF 5 REFERENCES
Covalent immobilization of α-amylase onto pHEMA microspheres: preparation and application to fixed bed reactor
Abstract Microspheres of poly(2-hydroxyethyl methacrylate) with and without cross-linker were prepared by suspension polymerization. As the amount of cross-linker increased, the equilibrium waterExpand
Effects of temperature on the hydrolysis of lactose by immobilized β‐galactosidase in a capillary bed reactor
TLDR
The effects of temperature on the hydrolysis of lactose by immobilized beta‐galactosidase and the temperature dependence of the mutarotation of galactose was effectively modelled by first‐order reversible kinetics. Expand
Identification, characterization, and partial purification of glucoamylase from Aspergillus niger (syn A. ficuum) NRRL 3135.
TLDR
The crude extracellular extract of Aspergillus niger NRRL 3135 contains glucoamylase (exo-1,4-alpha-D-glucanohydrolase, EC 3.2), a glycoprotein, which was purified 7-fold by ion-exchange chromatography, chromatofocusing, and conconavalin A affinity chromatography. Expand
Flow injection analysis
TLDR
This paper presents a meta-review of the Flow--Injection Literature and discusses theoretical Aspects of FIA Techniques, components of An FIA Apparatus, and Experimental Techniques and FIA Exercises. Expand