Determination of DNase activity by degradation of ethidium bromide-DNA complexes using a fluorescence plate reader.

@article{Vogel2015DeterminationOD,
  title={Determination of DNase activity by degradation of ethidium bromide-DNA complexes using a fluorescence plate reader.},
  author={Benjamin Vogel and Stefan Frantz},
  journal={Analytical biochemistry},
  year={2015},
  volume={471},
  pages={
          73-9
        }
}
The long known toxicity of free chromatin mediated by histones regained attention after discovery of neutrophil extracellular traps (NETs). Free histones from necrotic cells or NETs can damage prokaryotic and eukaryotic cells and are responsible for the aggravation of a growing list of diseases. DNases degrade the toxic chromatin polymer to nucleosomes and efficiently reduce local high histone concentrations. Therefore, DNase activity as a biomarker is of growing interest in basic and clinical… CONTINUE READING
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