Determination of D-penicillamine in serum by fluorescence derivatization and liquid column chromatography.


A simple and fast method for the determination of D-penicillamine in serum is described. The analysis is based o a fluorescence derivatization of the sulfhydryl group combined with a reversed-phase liquid chromatograhic separation and fluorescence detection. Before derivatization the serum proteins are precipitated with ethanol and removed by centrifugation… (More)


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