Determination of D-alanine in the rat central nervous system and periphery using column-switching high-performance liquid chromatography.

@article{Morikawa2003DeterminationOD,
  title={Determination of D-alanine in the rat central nervous system and periphery using column-switching high-performance liquid chromatography.},
  author={Akiko Morikawa and Kenji Hamase and Kiyoshi Zaitsu},
  journal={Analytical biochemistry},
  year={2003},
  volume={312 1},
  pages={66-72}
}
A column-switching chiral HPLC system for the determination of minute amounts of D-Ala in mammalian tissues has been established. D-Ala and its L-enantiomer are purified as a DL mixture on a micro-ODS column after precolumn fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole and are introduced to a chiral column to determine each enantiomer. The calibration curve of D-Ala spiked into a rat cerebellum sample is linear from 5 to 5000 fmol with a correlation coefficient of 1… CONTINUE READING
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