• Corpus ID: 87751574

Determination of Chitinase Activity of Pythium guiyangense Su and the Cloning and Analysis of A Fragment of the Chitinase Gene

@article{Xiaoqing2008DeterminationOC,
  title={Determination of Chitinase Activity of Pythium guiyangense Su and the Cloning and Analysis of A Fragment of the Chitinase Gene},
  author={Sun Xiaoqing},
  journal={Journal of Guiyang Medical College},
  year={2008}
}
  • Sun Xiaoqing
  • Published 2008
  • Biology
  • Journal of Guiyang Medical College
Objective: To test the chitinase activities produced by Pythium guiyangense Su, and to clone and sequence a fragment of the chitinase gene. Methods: 3,5-dinitrosalicylic acid chromatometry was used to test chitinase activities; DNA fragment was obtained with PCR technique using primers designed following conservative areas of chitinases of other organisms and sequenced. Results: It was demonstrated that P. guiyangens can produce chitinase after the induction of chitin-like substances, and the…