Determination of Binding Constant and Stoichiometry for Antibody-Antigen Interaction with Surface Plasmon Resonance

  title={Determination of Binding Constant and Stoichiometry for Antibody-Antigen Interaction with Surface Plasmon Resonance},
  author={Shiming Lin and Adam Shih-Yuan Lee and Chih-Chen Trista Lin and Chih-Kung Lee},
  journal={Current Proteomics},
A surface plasmon resonance (SPR) biosensor technology has recently been applied biochemically and clinically to the study of immunologic recognition and the evaluation of binding parameters for various interactions between antibodies (Abs) and antigens (Ags) at liquid-solid interface. The simple interaction between hapten and Ab fragment, e.g., variable single-chain fragment and antigen- binding fragment, can be described sufficiently by a 1:1 stoichiometry in SPR. However, the determination… 

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