Determination of Antineoplastic Activity of Rohituka, Aphanamixis Polystachya (Wall) RN Parker in Hela Cells: Correlation with Clonogenicity and DNA Damage

  title={Determination of Antineoplastic Activity of Rohituka, Aphanamixis Polystachya (Wall) RN Parker in Hela Cells: Correlation with Clonogenicity and DNA Damage},
  author={Ganesh Ch and R Jagetia and Venkatasubbaiah A. Venkatesha},
  journal={International Journal of Complementary and Alternative Medicine},
The antineoplastic activity of chloroform stem bark extract of rohituka, Aphanamixis polystachya (APE) used traditionally to treat spleen and liver tumors was evaluated in cultured HeLa cells by clonogenic and micronucleus assays. Treatment of HeLa cells with 0, 5, 10, 25, 50, 75 or 100µg/ml APE for different times caused a concentration-dependent reduction in the cell survival up to 6 h post-treatment (p<0.005) followed by a non-significant decline in the cells treated with APE for 24 h… 

Figures and Tables from this paper

Assessment of molecular DNA damage by comet assay in HeLa cells exposed to rohituka, Aphanamixis polystachya (wall.) R.N. Parker

It is demonstrated that rohituka triggers DNA damage in HeLa cells at molecular level that may be the major cause of reduced clonogenicity and growth kinetics.


The present study indicates that HNA exerted the cytotoxic effect on HeLa cells and recued the cell survival and this effect of HNA may be due to the alleviated level of the GSH, GST, catalase and SOD.

Evaluation of Antimicrobial, Thrombolytic and CNS-Depressant Activities of Three Medicinal Plants Available in Bangladesh

The aim of the present study was to explore antimicrobial, thrombolytic and CNS-depressant activity of three medicinal plants Plumbago zeylanica, Trewia nudiflora and Aphanamixis polystachya

The Isoquinoline Alkaloid Berberine Augments Radiation Effect by Enhancingthe DNA Damage at Molecular Level in HeLa Cells Irradiated with VariousDoses of ó-Radiation: Correlation Between DNA Damage and Clonogenicity

Berberine is a potent DNA damaging agent and could enhance radiation damage during cancer treatment in clinical conditions and clonogenicity of cells is directly related to the ability of berberine to inflict damage to DNA.

Anticancer activity of Helicia nilagirica bedd in mice transplanted with Dalton’s lymphoma

The cancer incidence has been increasing at an alarming rate and with modern diagnostic techniques more cancer cases are coming to light than ever before, and the figure is suspected to increase by five folds in the year 2025.


This review mainly focuses on medicinal plants Rosmarinus officinalis, Solanum nigrum, Kaffir lime, and Garcinia nujiangensis used in the treatment of cervical cancer and the secondary metabolites derived from different plant sources.



Preclinical Determination of the Anticancer Activity of Rohituka ( Aphanamixis polystachya ) in Ehrlich Ascites Tumor-Bearing Mice

It is indicated that APE treatment caused a dose dependent retardation in the tumor mass and regressed tumors even in the late stages of tumor development, which may be due to increased lipid peroxidation and reduction in the activities of antioxidant enzymes.

Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin

AME did reduce the cytotoxic and genotoxic effects of DOX and may be useful in clinical setup to reduce DOX-induced toxicity, and Assessment of DNA damage by micron nuclei assay showed that 25 µg/ml AME reduced the micronuclei frequency to a maximum extent.

Treatment of mice with stem bark extract of Aphanamixis polystachya reduces radiation-induced chromosome damage

This study demonstrates that EAP protects mouse bone marrow cells against radiation-induced chromosomal aberrations and this reduction in radiation- induced chromosome damage may be due to free radical scavenging and reduction in lipid peroxidation.

Assessment of micronucleus induction in murine SCCVII cells treated with various anticancer agents.

It was concluded that the cytokinesis-block micronucleus assay can be used to assess sensitivity of at least some tumor cells to appropriate concentrations of various chemotherapeutic agents.

Cytotoxicity Effects of Amoora rohituka and chittagonga on Breast and Pancreatic Cancer Cells

Rhituka and chittagonga, fractionated with petroleum ether, dichloromethane, and ethanol, were explored for their anticancer potential against two breast cancer and three pancreatic cancer cells and the extracts were not cytotoxic to normal fibroblast Hs68 cells, which may be correlated to the specificity of Amoora extracts in targeting cancerous cells.

Evaluation of Cytotoxic Effects of Dichloromethane Extract of Guduchi (Tinospora cordifolia Miers ex Hook F & THOMS) on Cultured HeLa Cells

  • G. JagetiaS. Rao
  • Biology, Chemistry
    Evidence-based complementary and alternative medicine : eCAM
  • 2006
The results suggest that the cytotoxic effect of TCE may be due to lipid peroxidation and release of LDH and decline in GST.

Modulation of antineoplastic activity of cyclophosphamide by Alstonia scholaris in the Ehrlich ascites carcinoma-bearing mice.

Alterations in the neoplastic activity of cyclophosphamide by the extract of Alstonia scholaris(ASE) were studied in mice transplanted with Ehrlich ascites carcinoma, as evident by the greater tumor remission, when compared with the concurrent doses of either drug alone.

Evaluation of the cytotoxic effect of the monoterpene indole alkaloid echitamine in‐vitro and in tumour‐bearing mice

It is demonstrated that echitamine chloride possessed anti‐tumour activity in‐vitro and in-vivo.

Novel Drug Amooranin Induces Apoptosis Through Caspase Activity in Human Breast Carcinoma Cell Lines

The results suggest that AMR is a novel drug having potential for clinical development against human malignancies and the mechanism of cell death associated with AMR cytotoxicity is investigated.