Determination of Amino Acids in Tunisian Animal Feedstuffs by HPLC-FLD

  title={Determination of Amino Acids in Tunisian Animal Feedstuffs by HPLC-FLD},
  author={Intissar Limam and Raouf Medimagh and Fayçal Hellal},
The amino acids supplemented to animal feedstuffs cannot be determined directly. Thus, we need to optimize the sample preparation step as well as the analytical method. In this study, two hydrolysis mode were performed, one using 6 mol.L-1 hydrochloric acid at 105°C for 24 h and the other based on microwave radiation-induced hydrolysis. Moreover, a chromatographic method was developed for simultaneous determination of methionine and lysine in feedstuffs, using conventional High Performance… CONTINUE READING


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