Determination of 8-iso-prostaglandin F(2alpha) in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS.

Abstract

Rapid and precise method for the determination of 8-iso-prostaglandin F(2alpha), an essential marker of the oxidative stress, in exhaled breath condensate (EBC) was developed. The protocol consisted of stable isotope dilution, immunoseparation combined with selective and sensitive LC-ESI-MS/MS operated in multiple reaction monitoring (MRM) mode. The imprecision of the developed method was below 8.8%, the parameter of mean inaccuracy was determined as <9.6% (0-250pg of 8-iso-prostaglandin F(2alpha)/ml EBC). The limit of detection (LOD) was 1 pg/ml EBC and limit of quantification (LOQ) 5 pg/ml EBC. A significant difference in 8-iso-prostaglandin F(2alpha) content between the group of asbestosis patients and healthy volunteers was found.

DOI: 10.1016/j.jchromb.2008.02.019

Cite this paper

@article{Syslova2008DeterminationO8, title={Determination of 8-iso-prostaglandin F(2alpha) in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS.}, author={Kamila Syslova and Petr Kacer and Marek Kuzma and Pavl{\'i}na Klus{\'a}{\vc}kov{\'a} and Zdena Fenclov{\'a} and Jindři{\vs}ka Lebedov{\'a} and Daniela Pelclov{\'a}}, journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences}, year={2008}, volume={867 1}, pages={8-14} }