Determination of 5-aminoimidazole-4-carboxamide in human plasma by ion-pair extraction and LC-MS/MS.

@article{Chen2007DeterminationO5,
  title={Determination of 5-aminoimidazole-4-carboxamide in human plasma by ion-pair extraction and LC-MS/MS.},
  author={Xi-jing Chen and Jing Jing and Weichao Ren and De-en Han and Ning Jing and Guang-ji Wang},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2007},
  volume={855 2},
  pages={140-4}
}
A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was established for the determination of 5-aminoimidazole-4-carboxamide (AICA) in human plasma. The method included a solvent extraction of AICA as an ion pair with 1-pentanesulfonate ion and a separation on a Hypersil ODS2 column with the mobile phase of methanol-water (68:32, v/v… CONTINUE READING