Determination of 3-nitrotyrosine by high-pressure liquid chromatography with a dual-mode electrochemical detector.

@article{Sodum2000DeterminationO3,
  title={Determination of 3-nitrotyrosine by high-pressure liquid chromatography with a dual-mode electrochemical detector.},
  author={Rama S. Sodum and Shobha Akerkar and Emerich S. Fiala},
  journal={Analytical biochemistry},
  year={2000},
  volume={280 2},
  pages={
          278-85
        }
}
3-Nitrotyrosine, a product of tyrosine nitration, is useful as a marker for the generation of reactive nitrogen oxide species with short half-lives such as peroxynitrite. A reverse-phase high-pressure liquid chromatographic method using a dual-mode electrochemical detector in series with a photodiode array detector has been developed to determine the levels of 3-nitrotyrosine in biological samples. The principle of this method involves reduction of 3-nitrotyrosine at an upstream gold amalgam… 
Determination of nitrotyrosine by HPLC-ECD and its application.
TLDR
In mice to which lipopolysaccharide was administered intraperitoneally, plasma 3-nitrotyrosine levels were elevated, corresponding to LPS dosage, and these findings suggest that the improved HPLC-ECD method can be used as a specific and sensitive assay of biological 3-NitroTYrosine and can be applied clinically.
3-Nitrotyrosine butyl ester: a novel derivative to assess tyrosine nitration in rat plasma by liquid chromatography-tandem mass spectrometry detection.
A novel, sensitive, and specific method is presented for the quantification of endogenous 3-nitrotyrosine in rat plasma based on isotope dilution liquid chromatography-electrospray ionization tandem
Simultaneous determination of 3-nitrotyrosine and tyrosine in plasma proteins of rats and assessment of artifactual tyrosine nitration.
Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological Samples and Comparison with Classical, Antibody-Based Methods
TLDR
Three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples are compared and the CoulArray quantification method seems to have some advantages regarding sensitivity and selectivity.
Disposition of protein-bound 3-nitrotyrosine in rat plasma analysed by a novel protocol for HPLC-ECD.
TLDR
The protocol is reproducible and suitable for analysing multiple clinical samples to study RNS production in vivo and the conditions for high performance liquid chromatography and electrochemical detection (HPLC-ECD) optimized.
Chromatographic and mass spectrometric methods for quantitative determination of 3-nitrotyrosine in biological samples and their application to human samples.
  • H. Ryberg, K. Caidahl
  • Biology, Chemistry
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2007
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 37 REFERENCES
Analytical methods for 3-nitrotyrosine as a marker of exposure to reactive nitrogen species: a review.
TLDR
Its role in biological systems may be more subtle with nitration of key tyrosine residues likely to profoundly affect cellular function such as signaling cascades and further advances are likely to be made in the localization of NTYR residues in peptide fragments using mass spectrometry.
Detection of 3-nitrotyrosine in human platelets exposed to peroxynitrite by a new gas chromatography/mass spectrometry assay.
TLDR
A new sensitive and specific assay was developed and applied for the quantitative determination of 3-nitrotyrosine in proteins of human platelets and showed excellent linearity and sensitivity.
Quantitation of protein-bound 3-nitrotyrosine and 3,4-dihydroxyphenylalanine by high-performance liquid chromatography with electrochemical array detection.
TLDR
High-performance liquid chromatography is used in conjunction with coulometric electrochemical array (HPLC-EC) detection to allow ultrasensitive determination of protein-bound 3-nitrotyrosine and 3, 4-dihydroxyphenylalanine (3-hydroxytyrosines) as specific in situ biomarkers of protein exposure to reactive nitrating and oxidizing species.
Inflammation and NO(X)-induced nitration: assay for 3-nitrotyrosine by HPLC with electrochemical detection.
TLDR
This assay offers a highly sensitive and quantitative approach for investigating the role of reactive byproducts of nitric oxide in the many pathological conditions and disease states associated with NO(X) exposure such as inflammation and smoking.
3-Nitrotyrosine in the proteins of human plasma determined by an ELISA method
TLDR
The presence of nitrotyrosine in LDL is consistent with previous reports implicating peroxynitrite in the oxidative modification of lipoproteins and the presence of a low concentration of oxidized LDL in the blood.
Isotope dilution mass spectrometric quantification of 3-nitrotyrosine in proteins and tissues is facilitated by reduction to 3-aminotyrosine.
TLDR
Quantifying 3-nitrotyrosine levels of tissues by stable isotope dilution gas chromatography/mass spectrometry should provide a powerful tool for exploring the impact of reactive nitrogen species on oxidative reactions in vivo.
Nitrotyrosine as a new marker for endogenous nitrosation and nitration of proteins.
Protein Nitration by Peroxynitrite: A Method for Monitoring Nitric Oxide Neurotoxicity
TLDR
The dose-dependent effects of peroxynitrite on the electrophoretic migration of a monoclonal antibody and the formation of nitrotyrosine from peroxyrosine roughly parallels the loss of tyrosine are studied.
...
1
2
3
4
...