Determination of [Mg2+]i – an update on the use of Mg2+-selective electrodes

  title={Determination of [Mg2+]i – an update on the use of Mg2+-selective electrodes},
  author={D. G{\"u}nzel and W. Schlue},
Since their invention, ion-selective microelectrodes have become an indispensable tool for investigations of intracellular ion regulation and transport. While highly selective sensors for all major intracellular monovalent ions have been available for decades, the development of sensors for divalent cations seems to have presented more difficulties. As ion-selective microelectrodes typically have time-constants in the range of 0.5 to several seconds they turned out to be inapt for the… Expand
Quantification of Mg2+, Ca2+ and H+ transport by the gastrointestinal tract of the goldfish, Carassius auratus, using the Scanning Ion-selective Electrode Technique (SIET)
An in vitro gut-sac technique and the scanning ion-selective electrode technique (SIET) were used to characterize Mg2+, Ca2+, and H+ transport at both the mucosal and serosal surfaces of non-everted and everted gastrointestinal tissues obtained from Carassius auratus, the first study to use SIET approaches to study ion transport in the gut of teleost fish. Expand
A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples.
These features, combined with the high fluorescence enhancement upon cation binding, and the possibility to be excited both in the UV and visible region, make DCHQ5 a valuable and versatile analytical tool for Mg assessment in biological samples. Expand
Determination of free Mg2 + in turbid samples with the coexistence of interfering Ca2 + using extended quantitative fluorescence model
Abstract Ratiometric fluorescence indicators are among the most popular fluorescence indicators due to their robustness to variations in probe loading, bleaching, optical path length, illuminationExpand
Monitoring magnesium degradation using microdialysis and fabric-based biosensors
This paper describes the development of a monitoring system capable of detecting the concentration of magnesium ions (Mg2+) released during the degradation of magnesium implants. The system consistsExpand
A modification, using polyaluminum chloride (PAC), was developed to remove the interference of phosphates from the colorimetric technique, and was found to produce reliable results within 10% of those results predicted by atomic absorption. Expand
A system for characterizing Mg corrosion in aqueous solutions using electrochemical sensors and impedance spectroscopy.
A corrosion characterization system that allows realtime monitoring of the solution soluble corrosion products OH-, Mg2+, and H2 during immersion tests commonly used to study the corrosion of Mg materials and has the potential to be useful in systematically testing and comparing the corrosion behavior of different Mg alloys, as well as protective coatings. Expand
Selective detection of Mg2+ ions via enhanced fluorescence emission using Au–DNA nanocomposites
The effectiveness of Au–DNA nanocomposites was verified by comparing the known concentration of Mg2+ ions in synthetic tap water and a real life sample of Gelusil, a widely used antacid medicine. Expand
A multi-ion fluorescent probe for Mg2+/Zn2+ based on a novel chromone-dendron Schiff base
Abstract A multi-ion fluorescent probe for Mg 2+ /Zn 2+ , 7-methoxychromone-3-carbaldehyde-(3′,4′,5′-tris(benzyloxy) benzoyl) hydrazone ( L ), based on a novel chromone-dendron Schiff base wasExpand
Investigating techniques to determine magnesium addition requirements for the operation of a struvite crystallization process
The recovery of phosphorus from wastewater has gathered strength due to its acceptance as a sustainable method for solving wastewater treatment plant struvite problems and the low global reserves ofExpand
The cationic composition and pH in the moulting fluid of Porcellio scaber (Crustacea, Isopoda) during calcium carbonate deposit formation and resorption
  • A. Ziegler
  • Chemistry, Medicine
  • Journal of Comparative Physiology B
  • 2007
The results suggest a possible role of magnesium ions in ACC formation, and a contribution of pH changes to CaCO3 precipitation and dissolution, in terrestrial isopods before moulting. Expand


Development of Magnesium-Ion-Selective Microelectrodes Based on a New Neutral Carrier ETHT 5504
Two types of magnesium-selective microelectrodes based on a new synthetic neutral carrier ETHT 5504 are presented. One type of microelectrode is applicable to the determination of intracellular freeExpand
Neutral carrier ion-selective microelectrodes for measurement of intracellular free calcium.
Electrodes showed a Nernstian response to [Ca2+] down to 1 micro M free concentration in 0.1 M KCl, and usually a useful response to below 100 nM Ca2+. Expand
Calibration of Mg(2+)ࢤselective macroelectrodes down to 1 mumol lߝ1 in intracellular and Ca(2+)ࢤcontaining extracellular solutions
Using Mg(2+)ࢤselective macroelectrodes based on the neutral carriers ETH 7025 and ETH 5506, methods were developed to determine accurately the apparent binding constant (Kapp) and purity, and henceExpand
Intracellular free magnesium and its regulation, studied in isolated ferret ventricular muscle with ion‐selective microelectrodes
Results in isolated ferret papillary muscles are not consistent with a Na(+)‐Mg2+ exchanger as being the main outward transport mechanism for Mg2- in this tissue. Expand
Novel magnesium ion-selective microelectrodes based on a neutral carrier
Two types of Mg2+-selective microelectrodes baed on a synthetic neutral carrier and containing poly-(vinyl chloride) (PVC) are described and Cyclohexanone is used for dissolving the PVC-containing membrane phases thus avoiding problems encountered with the much more volatile tetrahydrofuran. Expand
Direct measurement of intracellular free magnesium in frog skeletal muscle using magnesium-selective microelectrodes.
Determinations in muscle fibers during stimulation at low frequency (5 Hz), and after fatigue development, showed no changes in the concentration of free cytosolic Mg2+. Expand
Potentiometric microelectrodes as sensor and detectors. Magnesium-selective electrodes as sensors, and hofmeister electrodes as detectors for histamine in capillary electrophoresis
Abstract In order to apply magnesium-selective microelectrodes to intra- and extracellular measurements, a careful optimisation of the membrane composition, and of the electrode geometry isExpand
Estimation of the upper limit of the free magnesium concentration measured with Mg-sensitive microelectrodes in ferret ventricular muscle: (1) use of the Nicolsky-Eisenman equation and (2) in calibrating solutions of the appropriate concentration.
A critical appraisal of the literature values for [Mg]i in muscle tissue suggests that values over 2 mmol/l were either unphysiological or too high for methodological reasons, and compares calibrating solutions containing the mean values for K and Na to those containing the lowest measured values. Expand
Use of Ion Selective Microelectrodes to Measure Intracellular Free Mg2
Mg2+ has been dubbed “the forgotten cation” (Brady et al. 1987) for although it is the second most common intracellular cation, the study of its homeostasis and its regulatory function in health andExpand
Intracellular alkalinization causes Mg2+ release from intracellular binding sites in leech Retzius neurones
It is concluded that an intracellular alkalinization might induce the release of Mg2+ from intrace Cellular pH and the membrane potential. Expand