Determination of [Mg2+]i – an update on the use of Mg2+-selective electrodes

@article{Gnzel2004DeterminationO,
  title={Determination of [Mg2+]i – an update on the use of Mg2+-selective electrodes},
  author={Dorothee G{\"u}nzel and Wolf-R. Schlue},
  journal={Biometals},
  year={2004},
  volume={15},
  pages={237-249}
}
Since their invention, ion-selective microelectrodes have become an indispensable tool for investigations of intracellular ion regulation and transport. While highly selective sensors for all major intracellular monovalent ions have been available for decades, the development of sensors for divalent cations seems to have presented more difficulties. As ion-selective microelectrodes typically have time-constants in the range of 0.5 to several seconds they turned out to be inapt for the… 
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References

SHOWING 1-10 OF 60 REFERENCES
Development of Magnesium-Ion-Selective Microelectrodes Based on a New Neutral Carrier ETHT 5504
Two types of magnesium-selective microelectrodes based on a new synthetic neutral carrier ETHT 5504 are presented. One type of microelectrode is applicable to the determination of intracellular free
Calibration of Mg(2+)ࢤselective macroelectrodes down to 1 mumol lߝ1 in intracellular and Ca(2+)ࢤcontaining extracellular solutions
Using Mg(2+)ࢤselective macroelectrodes based on the neutral carriers ETH 7025 and ETH 5506, methods were developed to determine accurately the apparent binding constant (Kapp) and purity, and hence
Intracellular free magnesium and its regulation, studied in isolated ferret ventricular muscle with ion‐selective microelectrodes
TLDR
Results in isolated ferret papillary muscles are not consistent with a Na(+)‐Mg2+ exchanger as being the main outward transport mechanism for Mg2- in this tissue.
Novel magnesium ion-selective microelectrodes based on a neutral carrier
TLDR
Two types of Mg2+-selective microelectrodes baed on a synthetic neutral carrier and containing poly-(vinyl chloride) (PVC) are described and Cyclohexanone is used for dissolving the PVC-containing membrane phases thus avoiding problems encountered with the much more volatile tetrahydrofuran.
Intracellular free magnesium in excitable cells: its measurement and its biologic significance.
TLDR
The roles of intracellular Mg2+ on cell function as well as four current techniques for measuring [Mg2 +]i are briefly reviewed.
Intracellular free magnesium in neurones of Helix aspersa measured with ion‐selective micro‐electrodes.
TLDR
Cytoplasmic free Mg2+ concentration was measured in identified neuronal cell bodies of the suboesophageal ganglia of Helix aspersa, using M g2+‐selective micro‐electrodes, and an outwardly directed extrusion mechanism must exist to keep [Mg2-]i low and constant, even in cells undergoing spike activity.
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