Determination of [Mg2+]i – an update on the use of Mg2+-selective electrodes

  title={Determination of [Mg2+]i – an update on the use of Mg2+-selective electrodes},
  author={Dorothee G{\"u}nzel and Wolf-R. Schlue},
Since their invention, ion-selective microelectrodes have become an indispensable tool for investigations of intracellular ion regulation and transport. While highly selective sensors for all major intracellular monovalent ions have been available for decades, the development of sensors for divalent cations seems to have presented more difficulties. As ion-selective microelectrodes typically have time-constants in the range of 0.5 to several seconds they turned out to be inapt for the… 
Quantification of Mg2+, Ca2+ and H+ transport by the gastrointestinal tract of the goldfish, Carassius auratus, using the Scanning Ion-selective Electrode Technique (SIET)
An in vitro gut-sac technique and the scanning ion-selective electrode technique (SIET) were used to characterize Mg2+, Ca2+, and H+ transport at both the mucosal and serosal surfaces of non-everted and everted gastrointestinal tissues obtained from Carassius auratus, the first study to use SIET approaches to study ion transport in the gut of teleost fish.
A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples.
These features, combined with the high fluorescence enhancement upon cation binding, and the possibility to be excited both in the UV and visible region, make DCHQ5 a valuable and versatile analytical tool for Mg assessment in biological samples.
A modification, using polyaluminum chloride (PAC), was developed to remove the interference of phosphates from the colorimetric technique, and was found to produce reliable results within 10% of those results predicted by atomic absorption.
Selective detection of Mg2+ ions via enhanced fluorescence emission using Au–DNA nanocomposites
The effectiveness of Au–DNA nanocomposites was verified by comparing the known concentration of Mg2+ ions in synthetic tap water and a real life sample of Gelusil, a widely used antacid medicine.
Investigating techniques to determine magnesium addition requirements for the operation of a struvite crystallization process
  • K. Fattah, D. Mavinic
  • Materials Science
    International Journal of Environmental Science and Technology
  • 2013
The recovery of phosphorus from wastewater has gathered strength due to its acceptance as a sustainable method for solving wastewater treatment plant struvite problems and the low global reserves of
The cationic composition and pH in the moulting fluid of Porcellio scaber (Crustacea, Isopoda) during calcium carbonate deposit formation and resorption
  • A. Ziegler
  • Biology
    Journal of Comparative Physiology B
  • 2007
The results suggest a possible role of magnesium ions in ACC formation, and a contribution of pH changes to CaCO3 precipitation and dissolution, in terrestrial isopods before moulting.
10 – Magnesium (Mg) corrosion: a challenging concept for degradable implants
: Degradable metals are breaking the current paradigm in biomaterial science to develop only corrosion-resistant metals. In particular, metals which consist of trace elements existing in the human


Development of Magnesium-Ion-Selective Microelectrodes Based on a New Neutral Carrier ETHT 5504
Two types of magnesium-selective microelectrodes based on a new synthetic neutral carrier ETHT 5504 are presented. One type of microelectrode is applicable to the determination of intracellular free
Calibration of Mg(2+)ࢤselective macroelectrodes down to 1 mumol lߝ1 in intracellular and Ca(2+)ࢤcontaining extracellular solutions
Using Mg(2+)ࢤselective macroelectrodes based on the neutral carriers ETH 7025 and ETH 5506, methods were developed to determine accurately the apparent binding constant (Kapp) and purity, and hence
Intracellular free magnesium and its regulation, studied in isolated ferret ventricular muscle with ion‐selective microelectrodes
Results in isolated ferret papillary muscles are not consistent with a Na(+)‐Mg2+ exchanger as being the main outward transport mechanism for Mg2- in this tissue.
Novel magnesium ion-selective microelectrodes based on a neutral carrier
Two types of Mg2+-selective microelectrodes baed on a synthetic neutral carrier and containing poly-(vinyl chloride) (PVC) are described and Cyclohexanone is used for dissolving the PVC-containing membrane phases thus avoiding problems encountered with the much more volatile tetrahydrofuran.
Intracellular free magnesium in excitable cells: its measurement and its biologic significance.
The roles of intracellular Mg2+ on cell function as well as four current techniques for measuring [Mg2 +]i are briefly reviewed.
Intracellular free magnesium in neurones of Helix aspersa measured with ion‐selective micro‐electrodes.
Cytoplasmic free Mg2+ concentration was measured in identified neuronal cell bodies of the suboesophageal ganglia of Helix aspersa, using M g2+‐selective micro‐electrodes, and an outwardly directed extrusion mechanism must exist to keep [Mg2-]i low and constant, even in cells undergoing spike activity.