Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry.

  title={Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry.},
  author={Yoshinori Itoh and F H Ma and H. Hoshi and Michiko Oka and Kumiko Noda and Y Ukai and Hirotatsu Kojima and Tetsuo Nagano and N Toda},
  journal={Analytical biochemistry},
  volume={287 2},
  • Y. ItohF. Ma N. Toda
  • Published 15 December 2000
  • Biology, Chemistry
  • Analytical biochemistry
A simple and sensitive assay and a cellular bioimaging method for nitric oxide (NO) were developed using a novel diaminofluorescein DAF-FM and its diacetate. DAF-FM is converted via an NO-specific mechanism to an intensely fluorescent triazole derivative. For the measurement of NO, the triazole derivative of DAF-FM was determined by reversed-phase high-performance liquid chromatography with fluorescence detection. In the presence of 1 microM DAF-FM, the concentrations of NOR-1, an NO donor, in… 

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