• Chemistry, Medicine
  • Published in
    The Journal of biological…
    1993

Determinants of catalytic activity and stability of carbonic anhydrase II as revealed by random mutagenesis.

@article{Krebs1993DeterminantsOC,
  title={Determinants of catalytic activity and stability of carbonic anhydrase II as revealed by random mutagenesis.},
  author={John F. Krebs and Carol A. Fierke},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 2},
  pages={
          948-54
        }
}
The functional importance of a conserved hydrophobic face in human carbonic anhydrase II (CAII), including amino acid residues 190-210, was investigated by random mutagenesis. The catalytic activity, inhibitor binding, and level of CAII expression in Escherichia coli of 57 single amino acid variants were measured revealing that the function of amino acids correlates with their secondary structure placement. Side chains of amino acids in beta-sheet structure are required for the formation of… CONTINUE READING

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