Determinants of DNA yield and quality from buccal cell samples collected with mouthwash.

  title={Determinants of DNA yield and quality from buccal cell samples collected with mouthwash.},
  author={Heather Spencer Feigelson and C. D{\'i}az Rodr{\'i}guez and Andreas S Robertson and Eric J Jacobs and Eugenia E. Calle and Yvonne A. Reid and Michael J. Thun},
  journal={Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology},
  volume={10 9},
Buccal cells are becoming an important source of genomic DNA in epidemiological studies, but little is known about the effect of different sampling conditions on DNA quality and yield. We used a mouthwash protocol to collect six daily buccal cell samples from 35 healthy volunteers. Twenty-four individuals (six men and 18 women) correctly completed the protocol and were included in paired analyses to determine whether "swish" time (30 s versus 60 s), toothbrushing before collection, or lag time… CONTINUE READING

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