Determinant of cistron specificity in bacterial ribosomes

  title={Determinant of cistron specificity in bacterial ribosomes},
  author={John Shine and Lynn Dalgarno},
The sequence of the 3′-terminus of 16S RNA from different bacteria has been determined. Complementarity relationships between this sequence and a purine-rich tract in the ribosome binding site of different bacterial mRNAs suggest that the 3′-end of 16S RNA determines the intrinsic capacity of ribosomes to translate a particular cistron. 

Growth-dependent changes in terminal heterogeneity involving 3′-adenylate of bacterial 16S ribosomal RNA

It is proposed that a short sequence of nucleotides in this region has a direct base-pairing role in both the initiation and termination of protein synthesis in bacterial ribosomes.

Proximity of mRNA5′-region and 18S rRNA in eukaryotic initiation complexes

Messenger RNA was covalently linked to 18S ribosomal RNA in eukaryotic 40S and 80S initiation complexes by photoreaction with an RNA cross-linking agent, 4′-substituted psoralen. The sites of

Expression of a Small Protein Encoded by the 3' Flanking Sequence of the Escherichia coli rnpB Gene

This unit begins from position +354,and is successively repeated almost 3.5 times. The sequencesadditionally include three repeated sets of overlapping cod-ing regions, which may generate small

A model for the expression of CaMV nucleic acid

  • R. Hull
  • Biology
    Plant Molecular Biology
  • 2004
Analysis of the sequence of CaMV full-length 35S transcript reveals two features which may relate to the translation of open regions I-V, including a region in the 5′ leader sequence which could act as a ribosome binding site and a sequence complementary to sequences which are found just upstream to the open regions.

A transcription terminator in the 5' non-coding region of the tyrosyl tRNA synthetase gene from Bacillus stearothermophilus.

The 5' non-coding region of the tyrosyl-tRNA synthetase gene (tyrS) of Bacillus stearothermophilus is 324 nucleotides long. It contains a premature terminator and a strong promoter: these were

Nucleotide sequence of the small double-stranded RNA segment of bacteriophage phi 6: novel mechanism of natural translational control

It is proposed that the translational coupling is the result of an inability of ribosomes to bind independently to two of the four genes in the lipid-containing bacteriophage phi 6.



Sequence of a Ribosome Binding Site in Bacteriophage Qβ-RNA

A sequence of twenty-six nucleotides which includes the initiation region of the coat protein cistron of Qβ bacteriophage has been isolated and determined. The region contains the sequence UUUGA.

Ribosome Binding Site of Qβ RNA Polymerase Cistron

IN conditions of polypeptide chain initiation, 70S ribosomes bind to intact bacteriophage RNA predominantly at the initiation region of the coat protein cistron1,2. The binding sites of the A protein

Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA

The initiator regions of the three cistrons of R17 bacteriophage RNA have been isolated and sequenced. All three regions contain a UGA triplet as well as the expected AUG and two contain the sequence

Bacteriophage Ms2 RNA: nucleotide sequence of the end of the a protein gene and the intercistronic region.

The sequence of an RNA segment of 160 nucleotides preceding the coat protein initiator AUG has been established. It contains the genetic information for the last 45 amino-acids of the A or maturation

Conserved terminal sequence in 18SrRNA may represent terminator anticodons.

The 3′-terminal octanucleotide sequences of 18S rRNA from yeast, Drosophila and rabbit reticulocytes are identical and complementary to known eukaryotic terminator codons suggesting a role for the3′-end of the small rRNA in the termination of protein synthesis.

Isolation and Sequence Analysis of a Ribosome-protected Fragment from Bacteriophage φX 174 DNA

The DNA sequence corresponding to the gene G spike protein initiation site has been determined by analysis of endonuclease IV and ‘Streptodornase’ digests. The sequence has many features in common

Localization of Qβ Maturation Cistron Ribosome Binding Site

The RNA of phage Qβ consists of three known cistrons coding for maturation protein, coat protein and the β subunit of the viral replicase and when ribosomes are bound to intact Qβ RNA they attach predominantly to one binding site, namely that at the beginning of the coat cistron.

Specificity in Bacterial Protein Synthesis : Role of Initiation Factors and Ribosomal Subunits

Ribosomes from E. coli initiate synthesis in vitro of all three of the proteins coded by phage f2, but ribosomes from B. stearothermophilus initiate synthesis of only one. The specificity of

Species Specificity of Polypeptide Chain Initiation

Ribosomes of B. stearothermophilus differ from those of Escherichia coli in initiating the synthesis of only one protein coded for by bacteriophage f2 RNA, irrespective of the reaction conditions,

Direct physical evidence for secondary structure in an isolated fragment of R17 bacteriophage mRNA

The region of R17 RNA protected from RNase digestion by coat protein assumes a stable, predictable secondary structure that is recognised by the protein when functioning as a translational srepressor.