Detergent screening of a G-protein-coupled receptor using serial and array biosensor technologies.

@article{Rich2009DetergentSO,
  title={Detergent screening of a G-protein-coupled receptor using serial and array biosensor technologies.},
  author={Rebecca L. Rich and Adam R Miles and Bruce K. Gale and David G. Myszka},
  journal={Analytical biochemistry},
  year={2009},
  volume={386 1},
  pages={98-104}
}
We describe the benefits and limitations of two biosensor approaches for screening solubilization conditions for G-protein-coupled receptors (GPCRs). Assays designed for a serial processing instrument (Biacore 2000/3000/T100) and an array platform (Biacore Flexchip) were used to examine how effectively 96 different detergents solubilized the chemokine receptor CCR5 while maintaining its binding activity for a conformationally sensitive Fab (2D7). Using the serial processing instrument, we were… CONTINUE READING

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