Detergent-mediated reconstitution of membrane proteins.

  title={Detergent-mediated reconstitution of membrane proteins.},
  author={Jaco C. Knol and Klaas A. Sjollema and Bert Poolman},
  volume={37 46},
The efficiency of reconstitution of the lactose transport protein (LacS) of Streptococcus thermophilus is markedly higher with Triton X-100 than with other detergents commonly employed to mediate the membrane insertion. To rationalize these differences, the lipid/detergent structures that are formed in the reconstitution process were studied by cryotransmission electron microscopy. Surprisingly, the two nonionic detergents Triton X-100 and n-dodecyl beta-D-maltoside (DDM) affected the liposome… CONTINUE READING