Detergent interaction with band 3, a model polytopic membrane protein.

@article{Casey1993DetergentIW,
  title={Detergent interaction with band 3, a model polytopic membrane protein.},
  author={Joseph R Casey and Reinhart A. F. Reithmeier},
  journal={Biochemistry},
  year={1993},
  volume={32 4},
  pages={1172-9}
}
The interaction of band 3, the 95-kDa anion-exchange protein of the human erythrocyte membrane, with a variety of nonionic detergents was studied. Band 3 dimers (Stokes radius = 76 A) prepared in octaethylene glycol monododecyl ether (C12E8) could be exchanged into a variety of detergents by size-exclusion high-performance liquid chromatography (HPLC), with complete removal of C12E8 from band 3 being confirmed using radiolabeled detergent. Critical micellar concentration (cmc) values… CONTINUE READING