Detergent extraction of herpes simplex virus type 1 glycoprotein D by zwitterionic and non-ionic detergents and purification by ion-exchange high-performance liquid chromatography.

Abstract

Detergents (surfactants) are the key reagents in the extraction and purification of integral membrane proteins. Zwitterionic and non-ionic detergents were used for the extraction of recombinant glycoprotein D (gD-1) of herpes simplex virus type 1 (HSV-1) from insect cells infected with recombinant baculovirus. The highest yield was obtained with the two… (More)

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@article{WellingWester1998DetergentEO, title={Detergent extraction of herpes simplex virus type 1 glycoprotein D by zwitterionic and non-ionic detergents and purification by ion-exchange high-performance liquid chromatography.}, author={Sytske Welling-Wester and Matty Feijlbrief and Danny G. A. M. Koedijk and Gjalt W. Welling}, journal={Journal of chromatography. A}, year={1998}, volume={816 1}, pages={29-37} }