Detection of weakly interacting anti-carbohydrate scFv phages using surface plasmon resonance.

@article{Astrm2006DetectionOW,
  title={Detection of weakly interacting anti-carbohydrate scFv phages using surface plasmon resonance.},
  author={Eva Astr{\"o}m and Sten Ohlson},
  journal={Journal of molecular recognition : JMR},
  year={2006},
  volume={19 4},
  pages={282-6}
}
Methods to characterise and confirm specificity of scFv displayed on phages are important during panning procedures, especially when selecting for antibody fragments with weak affinities in the millimole to micromole range. In this report the surface plasmon resonance (SPR) biosensor was used to study and verify specificity of phages displaying weak anti-carbohydrate scFvs. The variable immunoglobulin light (VL) and heavy (VH) chain genes of the weak monoclonal antibody 39.5 were amplified and… CONTINUE READING