Detection of water toxicity using cytochrome P450 transgenic zebrafish as live biosensor: for polychlorinated biphenyls toxicity.

@article{Hung2012DetectionOW,
  title={Detection of water toxicity using cytochrome P450 transgenic zebrafish as live biosensor: for polychlorinated biphenyls toxicity.},
  author={Karen W Y Hung and Miranda F K Suen and Yong Fu Chen and Hung Bo Cai and Zhi Xian Mo and Ken Kin-Lam Yung},
  journal={Biosensors & bioelectronics},
  year={2012},
  volume={31 1},
  pages={548-53}
}
Cytochrome P450 (CYPs) is significant in degradation of endogenous substrates and detoxification of carcinogens, therefore it is a biomarker for assessment of polycyclic aromatic hydrocarbons (PAHs) level in aquatic environment. In the present study, a transgenic line of zebrafish had been generated using a CYP-green fluorescence protein (CYP-GFP) construct, driven by CYP1A1 promoter. Polychlorinated biphenyls (PCBs) were used as toxicant, in concentrations of 0.02 μg/ml, 0.04 μg/ml, 0.08 μg/ml… CONTINUE READING