Detection of viruses in nasal swab samples from horses with acute, febrile, respiratory disease using virus isolation, polymerase chain reaction and serology.

@article{Dynon2007DetectionOV,
  title={Detection of viruses in nasal swab samples from horses with acute, febrile, respiratory disease using virus isolation, polymerase chain reaction and serology.},
  author={Kemperley Dynon and Wesley D Black and Nino P. Ficorilli and Carol Anne Hartley and Michael J. Studdert},
  journal={Australian veterinary journal},
  year={2007},
  volume={85 1-2},
  pages={46-50}
}
OBJECTIVE To examine the association of viruses with acute febrile respiratory disease in horses. Design Nasal swab and serum samples were collected from 20 horses with acute febrile upper respiratory disease that was clinically assessed to have a viral origin. METHODS Each of the samples was inoculated onto equine fetal kidney, RK13 and Vero cell cultures, and viral nucleic acid was extracted for polymerase chain reaction (PCR) or reverse transcription PCR. PCR primers were designed to… CONTINUE READING
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