Detection of virus and cellular-determined antigens in situ using [125I]protein A and autoradiography.

Abstract

The present paper describes the use of [125I]Protein A, isolated from Staphylococcus aureus, in detecting antigen-antibody complexes by autoradiography on single cells. the method is relatively quick, reproducible, potentially more sensitive than immunofluorescence, and should be useful in combination with conventional radioimmuno-assays. We have used it to detect the cellular expression of IgM, kappa, lambda, and beta 2-microglobulin, as well as the expression of Epstein-Barr Virus (EBV)-associated antigens expressed in human lymphoblastoid cell lines.

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@article{Moar1979DetectionOV, title={Detection of virus and cellular-determined antigens in situ using [125I]protein A and autoradiography.}, author={Magnus Moar and Antony Rosen and Gunnar O. Klein}, journal={Journal of immunological methods}, year={1979}, volume={30 3}, pages={219-29} }