Detection of viral microRNA with S1 nuclease protection assay.

Abstract

Mammalian host cells and their viral pathogens express and make use of short noncoding RNA molecules to control the infectious cycle. In order to understand their physiological role, it is necessary to develop tools for detection and quantification of these molecules. Here, we present a simple, specific, and very sensitive protocol using short radioactive… (More)
DOI: 10.1007/978-1-61779-037-9_10

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