Detection of viable Listeria monocytogenes with a 5' nuclease PCR assay.

@article{Norton1999DetectionOV,
  title={Detection of viable Listeria monocytogenes with a 5' nuclease PCR assay.},
  author={Dawn M Norton and Carl A. Batt},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 5},
  pages={
          2122-7
        }
}
A 5' nuclease assay has been developed to detect viable Listeria monocytogenes. The assay targets the hemolysin A (hlyA) transcript, which is found only in L. monocytogenes. The single-tube, reverse transcriptase (RT), fluorogenic probe-based assay was formatted by using Tth DNA polymerase whose activity was modulated by using the manganese-chelating morpholinepropanesulfonic acid (MOPS) buffer. This assay was quantitative over a 3-log-unit range of template concentrations when tested with an… CONTINUE READING