Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode.

@article{Steen2001DetectionOT,
  title={Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode.},
  author={Hanno Steen and Bernhard K{\"u}ster and Marta Fernandez and Akhilesh Pandey and Matthias Mann},
  journal={Analytical chemistry},
  year={2001},
  volume={73 7},
  pages={1440-8}
}
Phosphorylation is a common form of protein modification. To understand its biological role, the site of phosphorylation has to be determined. Generally, only limited amounts of phosphorylated proteins are present in a cell, thus demanding highly sensitive procedures for phosphorylation site determination. Here, a novel method is introduced which enables the localization of tyrosine phosphorylation in gel-separated proteins in the femtomol range. The method utilizes the immonium ion of… CONTINUE READING

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